Immobilization of Glucose Oxidase on a Novel Crosslinked Chitosan Support Grafted with L-Lysine Spacers
نویسندگان
چکیده
A novel L-lysine modified semi-crosslinked chitosan resin (LMCCR) was synthesized and demonstrated to be a promising enzyme support by studying the enzymatic properties of glucose oxidases (GODs) immobilized on it. The prepared LMCCR beads have large specific surface and excellent chemical stability. The insertion of flexible L-lysine spacers between chitosan backbone and the immobilized GODs (I-GODs) increases the enzymes’ activity and improves their affinity towards the substrate. Repetitive uses demonstrated that the LMCCR-immobilized GODs have excellent operational stability and reusability. Moreover, the enzyme activity at varied temperatures and pH indicated that the GODs immobilized on LMCCR have good thermostability and pH stability.
منابع مشابه
Study on Different Molecular Weights of Chitosan as an Immobilization Matrix for a Glucose Biosensor
Two chitosan samples (medium molecular weight (MMCHI) and low molecular weight (LMCHI)) were investigated as an enzyme immobilization matrix for the fabrication of a glucose biosensor. Chitosan membranes prepared from acetic acid were flexible, transparent, smooth and quick-drying. The FTIR spectra showed the existence of intermolecular interactions between chitosan and glucose oxidase (GOD). H...
متن کاملRemoval of Malachite Green by Using Immobilized Glucose Oxidase Onto Silica Nanostructure-Coated Silver Metal-Foam
Enzymes Immobilization onto different types of the substrate could be helpful in various applicationsof biomedical devices and biosensors. Enzyme activity and stability could be affected by support andmethod of immobilization. In this study, the silver metal foam was successfully synthesized by thesoft-shell method and then was coated with silica. Then, glucose oxidase (GOx) immobilized on nonc...
متن کاملImmobilization of Glucose oxidase on Meso-porous Glass-ceramic with the Skeleton of CaTi4(PO¬4)6
Microporous glass ceramic with skeleton of CaTi4(PO¬4)6 with average pore size of 12.7 nm has been synthesized and used as a carrier of glucose oxidase. The glass ceramic was prepared by controlled heat treatment of glass samples, which causes the phase separation in their structure and creates CaTi4(PO¬4)6 and β-Ca3(PO4)2 phases. The β-Ca3(PO4)2 phase was dissolved by soaking the glass ceramic...
متن کاملNitrogen-Doped Carbon Dots as A New Substrate for Sensitive Glucose Determination
Nitrogen-doped carbon dots are introduced as a novel substrate suitable for enzyme immobilization in electrochemical detection metods. Nitrogen-doped carbon dots are easily synthesised from polyacrylamide in just one step. With the help of the amino group on chitosan, glucose oxidase is immobilized on nitrogen-doped carbon dots-modified carbon glassy electrodes by amino-carboxyl reactions. The ...
متن کاملKinetics and Isotherm Studies of the Immobilized Lipase on Chitosan Support
The kinetics and isotherm studies of the immobilized lipase and the mechanism of immobilization on chitosan beads and activated chitosan beads with glutaraldehyde were investigated. The effect of glutaraldehyde on porosity of chitosan was evaluated by FESEM analysis. It was observed that the porosity of the carrier which has activated by glutaraldehyde was substantially increased. The validity ...
متن کامل